Mimotopes identify conformational B-cell epitopes on the two major house dust mite allergens Der p 1 and Der p 2.

Publication Type:

Journal Article

Source:

Molecular Immunology, Volume 45, Issue 5, p.1308-17 (2008)

Keywords:

Animals, Antigens, Dermatophagoides, Arthropod Proteins, Cysteine Endopeptidases, Epitopes, B-Lymphocyte, Humans, Hypersensitivity, Immunoglobulin E, Immunotherapy, Molecular Mimicry, Peptide Library, Protein Conformation

Abstract:

House dust mite allergy occurs in 10-20% of the population. Improvement of the present immunotherapy requires detailed knowledge about the structure of the allergens. Mimotopes selected from phage peptide libraries imitate the conformational epitopes of a natural allergen. The aim of our study was to generate epitope mimics for the two major allergens of house dust mite. When the monoclonal anti-Der p 1 and anti-Der p 2 antibodies were used for biopannings, mimotopes were selected which bound also specific IgE from human allergic patients' sera. The conformational matching of these mimotopes on the 3D structure of the natural allergens determined discontinuous epitopes in both cases, representing conformational B-cell epitopes relevant for binding of human IgE. Therefore, these mimotopes are potential candidates for the directed induction of blocking antibodies and epitope-specific immunotherapy of mite allergy.

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